应用双向电泳分析2株不同毒力的副猪嗜血杆菌蛋白质表达的差异

doi:10.11843/j.issn.0366-6964.2015.03.022

Abstract

Abstract:

Haemophilus parasuis was one of the common bacterial disease pathogen of pigs，clinically characterized by fibrinous polyserositis，fibrinous arthritis and meningitis.It had importance significance that the expressed proteins of different virulent H.parasuis strains were profiled by the comparative proteomic approach to reveal the mechanism of pathogenisis.We chose 2 different virulent stains，stain A and stain B，of H.parasuis serotype 4 for comparative analysis，10 differently expressed proteins were analyzed and identified by 2-DE and MALDI-TOF-MS.In all proteins differentially expressed，3 protein spots were upregulated in strain A，they were glutamyl-tRNA synthetase，cysteine synthase and hypothetical protein，and 7 protein spots were upregulated in strain B included YaeT protein（omp85），CTP synthetase，aspartyl-tRNA synthetase（3 spots），uridylate kinase，protein-export chaperone SecB and so on.The function of expressed proteins mainly consisted of protein biosynthesis，amino-acid biosynthesis and pyrimidine biosynthesis.These results further enrich the information of virulence factors and pathogenic mechanisms of the H.parasuis strains，and also lay a foundation for study of immune proteomic.

CLC Number:

S854.4

WANG Chen-yan，WANG Long-bai，CHE Yong-liang，WU Xue-min，CHEN Ru-jing，SHAO Liang-ping，ZHOU Lun-jiang. Differential Analysis of Expressed Proteins between Different Virulent Haemophilus parasuis by Two-dimensional Polyacrylamide Gel Electrophoresis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(3): 503-508.

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Differential Analysis of Expressed Proteins between Different Virulent Haemophilus parasuis by Two-dimensional Polyacrylamide Gel Electrophoresis

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